rabbit anti-dynorphin polyclonal antibody Search Results


goat anti dynorphin polyclonal antibody  (Santa Cruz Biotechnology)
95
Santa Cruz Biotechnology goat anti dynorphin polyclonal antibody
Ambulation was recorded for 180 min after injection of a single dose of cocaine (25 mg/kg, i.p.) or vehicle in fb-A 2A R KO (n = 12, A ) and in st-A 2A R KO (n = 9, B ) mice and their WT littermates (n = 8–12). The arrow indicates the time of injection and the data are mean ± SEM; *p<0.05, comparing fb-A 2A R KO and st-A 2A R KO groups to their corresponding WT group using two-way ANOVA and a post hoc Bonferroni test. ( C ) Cocaine-induced c-Fos expression in the striatum of fb-A 2A R KO (n = 12) and st-A 2A R KO (n = 9) and their corresponding WT littermates (n = 8–12) # p<0.05, comparing to corresponding wild-types with cocaine treatment, two-way ANOVA post hoc Bonferroni test. ( D ) Representative co-immunostaining of c-Fos with <t>dynorphin.</t> Black arrows indicate neurons co-stained with dynorphin and c-Fos; white arrow heads indicate neurons stained with dynorphin only (greyish brown) and black arrow heads indicate neurons stained with c-Fos (reddish brown). Scale bar = 25 µm.
Goat Anti Dynorphin Polyclonal Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+anti-dynorphin+polyclonal+antibody/IGF-IR%CE%B1+Antibody/pmc03842921-126-9-15
Average 95 stars, based on 1 article reviews
goat anti dynorphin polyclonal antibody - by Bioz Stars, 2026-09
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rabbit anti-dynorphin a  (Bachem)
90
Bachem rabbit anti-dynorphin a
Ambulation was recorded for 180 min after injection of a single dose of cocaine (25 mg/kg, i.p.) or vehicle in fb-A 2A R KO (n = 12, A ) and in st-A 2A R KO (n = 9, B ) mice and their WT littermates (n = 8–12). The arrow indicates the time of injection and the data are mean ± SEM; *p<0.05, comparing fb-A 2A R KO and st-A 2A R KO groups to their corresponding WT group using two-way ANOVA and a post hoc Bonferroni test. ( C ) Cocaine-induced c-Fos expression in the striatum of fb-A 2A R KO (n = 12) and st-A 2A R KO (n = 9) and their corresponding WT littermates (n = 8–12) # p<0.05, comparing to corresponding wild-types with cocaine treatment, two-way ANOVA post hoc Bonferroni test. ( D ) Representative co-immunostaining of c-Fos with <t>dynorphin.</t> Black arrows indicate neurons co-stained with dynorphin and c-Fos; white arrow heads indicate neurons stained with dynorphin only (greyish brown) and black arrow heads indicate neurons stained with c-Fos (reddish brown). Scale bar = 25 µm.
Rabbit Anti Dynorphin A, supplied by Bachem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+anti-dynorphin+polyclonal+antibody/rabbit+antiserum+against+dynorphin+b/us09506068-508-76-79
Average 90 stars, based on 1 article reviews
rabbit anti-dynorphin a - by Bioz Stars, 2026-09
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polyclonal anti dynorphin a antibody  (Bio-Rad)
80
Bio-Rad polyclonal anti dynorphin a antibody
Ambulation was recorded for 180 min after injection of a single dose of cocaine (25 mg/kg, i.p.) or vehicle in fb-A 2A R KO (n = 12, A ) and in st-A 2A R KO (n = 9, B ) mice and their WT littermates (n = 8–12). The arrow indicates the time of injection and the data are mean ± SEM; *p<0.05, comparing fb-A 2A R KO and st-A 2A R KO groups to their corresponding WT group using two-way ANOVA and a post hoc Bonferroni test. ( C ) Cocaine-induced c-Fos expression in the striatum of fb-A 2A R KO (n = 12) and st-A 2A R KO (n = 9) and their corresponding WT littermates (n = 8–12) # p<0.05, comparing to corresponding wild-types with cocaine treatment, two-way ANOVA post hoc Bonferroni test. ( D ) Representative co-immunostaining of c-Fos with <t>dynorphin.</t> Black arrows indicate neurons co-stained with dynorphin and c-Fos; white arrow heads indicate neurons stained with dynorphin only (greyish brown) and black arrow heads indicate neurons stained with c-Fos (reddish brown). Scale bar = 25 µm.
Polyclonal Anti Dynorphin A Antibody, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 80/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+anti-dynorphin+polyclonal+antibody/Rabbit+anti+Human+Dynorphin+A+(aa1-17)/pmc06772802-144-43-48
Average 80 stars, based on 1 article reviews
polyclonal anti dynorphin a antibody - by Bioz Stars, 2026-09
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anti-dynorphin b  (Bachem)
90
Bachem anti-dynorphin b
Ambulation was recorded for 180 min after injection of a single dose of cocaine (25 mg/kg, i.p.) or vehicle in fb-A 2A R KO (n = 12, A ) and in st-A 2A R KO (n = 9, B ) mice and their WT littermates (n = 8–12). The arrow indicates the time of injection and the data are mean ± SEM; *p<0.05, comparing fb-A 2A R KO and st-A 2A R KO groups to their corresponding WT group using two-way ANOVA and a post hoc Bonferroni test. ( C ) Cocaine-induced c-Fos expression in the striatum of fb-A 2A R KO (n = 12) and st-A 2A R KO (n = 9) and their corresponding WT littermates (n = 8–12) # p<0.05, comparing to corresponding wild-types with cocaine treatment, two-way ANOVA post hoc Bonferroni test. ( D ) Representative co-immunostaining of c-Fos with <t>dynorphin.</t> Black arrows indicate neurons co-stained with dynorphin and c-Fos; white arrow heads indicate neurons stained with dynorphin only (greyish brown) and black arrow heads indicate neurons stained with c-Fos (reddish brown). Scale bar = 25 µm.
Anti Dynorphin B, supplied by Bachem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+anti-dynorphin+polyclonal+antibody/dynorphin+b/pmc03606903-48-20-24
Average 90 stars, based on 1 article reviews
anti-dynorphin b - by Bioz Stars, 2026-09
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rabbit anti dynorphin a  (Peninsula Laboratories)
86
Peninsula Laboratories rabbit anti dynorphin a
Ambulation was recorded for 180 min after injection of a single dose of cocaine (25 mg/kg, i.p.) or vehicle in fb-A 2A R KO (n = 12, A ) and in st-A 2A R KO (n = 9, B ) mice and their WT littermates (n = 8–12). The arrow indicates the time of injection and the data are mean ± SEM; *p<0.05, comparing fb-A 2A R KO and st-A 2A R KO groups to their corresponding WT group using two-way ANOVA and a post hoc Bonferroni test. ( C ) Cocaine-induced c-Fos expression in the striatum of fb-A 2A R KO (n = 12) and st-A 2A R KO (n = 9) and their corresponding WT littermates (n = 8–12) # p<0.05, comparing to corresponding wild-types with cocaine treatment, two-way ANOVA post hoc Bonferroni test. ( D ) Representative co-immunostaining of c-Fos with <t>dynorphin.</t> Black arrows indicate neurons co-stained with dynorphin and c-Fos; white arrow heads indicate neurons stained with dynorphin only (greyish brown) and black arrow heads indicate neurons stained with c-Fos (reddish brown). Scale bar = 25 µm.
Rabbit Anti Dynorphin A, supplied by Peninsula Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+anti-dynorphin+polyclonal+antibody/against+antiserum+polyclonal+rabbit+rat+urp/10__1523_slash_jneurosci__20___20___07798__2000-56-15-18
Average 86 stars, based on 1 article reviews
rabbit anti dynorphin a - by Bioz Stars, 2026-09
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rabbit anti dynorphin a antibody 1 13 porcine  (Cell Signaling Technology Inc)
95
Cell Signaling Technology Inc rabbit anti dynorphin a antibody 1 13 porcine
Ambulation was recorded for 180 min after injection of a single dose of cocaine (25 mg/kg, i.p.) or vehicle in fb-A 2A R KO (n = 12, A ) and in st-A 2A R KO (n = 9, B ) mice and their WT littermates (n = 8–12). The arrow indicates the time of injection and the data are mean ± SEM; *p<0.05, comparing fb-A 2A R KO and st-A 2A R KO groups to their corresponding WT group using two-way ANOVA and a post hoc Bonferroni test. ( C ) Cocaine-induced c-Fos expression in the striatum of fb-A 2A R KO (n = 12) and st-A 2A R KO (n = 9) and their corresponding WT littermates (n = 8–12) # p<0.05, comparing to corresponding wild-types with cocaine treatment, two-way ANOVA post hoc Bonferroni test. ( D ) Representative co-immunostaining of c-Fos with <t>dynorphin.</t> Black arrows indicate neurons co-stained with dynorphin and c-Fos; white arrow heads indicate neurons stained with dynorphin only (greyish brown) and black arrow heads indicate neurons stained with c-Fos (reddish brown). Scale bar = 25 µm.
Rabbit Anti Dynorphin A Antibody 1 13 Porcine, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+anti-dynorphin+polyclonal+antibody/CD71+XP+Rabbit+mAb/pmc06618541-164-33-30
Average 95 stars, based on 1 article reviews
rabbit anti dynorphin a antibody 1 13 porcine - by Bioz Stars, 2026-09
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rabbit anti–met-enkephalin ab  (Bachem)
90
Bachem rabbit anti–met-enkephalin ab
Ambulation was recorded for 180 min after injection of a single dose of cocaine (25 mg/kg, i.p.) or vehicle in fb-A 2A R KO (n = 12, A ) and in st-A 2A R KO (n = 9, B ) mice and their WT littermates (n = 8–12). The arrow indicates the time of injection and the data are mean ± SEM; *p<0.05, comparing fb-A 2A R KO and st-A 2A R KO groups to their corresponding WT group using two-way ANOVA and a post hoc Bonferroni test. ( C ) Cocaine-induced c-Fos expression in the striatum of fb-A 2A R KO (n = 12) and st-A 2A R KO (n = 9) and their corresponding WT littermates (n = 8–12) # p<0.05, comparing to corresponding wild-types with cocaine treatment, two-way ANOVA post hoc Bonferroni test. ( D ) Representative co-immunostaining of c-Fos with <t>dynorphin.</t> Black arrows indicate neurons co-stained with dynorphin and c-Fos; white arrow heads indicate neurons stained with dynorphin only (greyish brown) and black arrow heads indicate neurons stained with c-Fos (reddish brown). Scale bar = 25 µm.
Rabbit Anti–Met Enkephalin Ab, supplied by Bachem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+anti-dynorphin+polyclonal+antibody/polyclonal+rabbit+anti+vp/pmc02631295-200-15-18
Average 90 stars, based on 1 article reviews
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guinea pig anti–β-endorphin ab  (Bachem)
90
Bachem guinea pig anti–β-endorphin ab
Ambulation was recorded for 180 min after injection of a single dose of cocaine (25 mg/kg, i.p.) or vehicle in fb-A 2A R KO (n = 12, A ) and in st-A 2A R KO (n = 9, B ) mice and their WT littermates (n = 8–12). The arrow indicates the time of injection and the data are mean ± SEM; *p<0.05, comparing fb-A 2A R KO and st-A 2A R KO groups to their corresponding WT group using two-way ANOVA and a post hoc Bonferroni test. ( C ) Cocaine-induced c-Fos expression in the striatum of fb-A 2A R KO (n = 12) and st-A 2A R KO (n = 9) and their corresponding WT littermates (n = 8–12) # p<0.05, comparing to corresponding wild-types with cocaine treatment, two-way ANOVA post hoc Bonferroni test. ( D ) Representative co-immunostaining of c-Fos with <t>dynorphin.</t> Black arrows indicate neurons co-stained with dynorphin and c-Fos; white arrow heads indicate neurons stained with dynorphin only (greyish brown) and black arrow heads indicate neurons stained with c-Fos (reddish brown). Scale bar = 25 µm.
Guinea Pig Anti–β Endorphin Ab, supplied by Bachem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+anti-dynorphin+polyclonal+antibody/guinea+pig+anti+vp/pmc02631295-199-18-34
Average 90 stars, based on 1 article reviews
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rabbit leucine enkephalin polyclonal antibody  (ImmunoStar inc)
90
ImmunoStar inc rabbit leucine enkephalin polyclonal antibody
Ambulation was recorded for 180 min after injection of a single dose of cocaine (25 mg/kg, i.p.) or vehicle in fb-A 2A R KO (n = 12, A ) and in st-A 2A R KO (n = 9, B ) mice and their WT littermates (n = 8–12). The arrow indicates the time of injection and the data are mean ± SEM; *p<0.05, comparing fb-A 2A R KO and st-A 2A R KO groups to their corresponding WT group using two-way ANOVA and a post hoc Bonferroni test. ( C ) Cocaine-induced c-Fos expression in the striatum of fb-A 2A R KO (n = 12) and st-A 2A R KO (n = 9) and their corresponding WT littermates (n = 8–12) # p<0.05, comparing to corresponding wild-types with cocaine treatment, two-way ANOVA post hoc Bonferroni test. ( D ) Representative co-immunostaining of c-Fos with <t>dynorphin.</t> Black arrows indicate neurons co-stained with dynorphin and c-Fos; white arrow heads indicate neurons stained with dynorphin only (greyish brown) and black arrow heads indicate neurons stained with c-Fos (reddish brown). Scale bar = 25 µm.
Rabbit Leucine Enkephalin Polyclonal Antibody, supplied by ImmunoStar inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+anti-dynorphin+polyclonal+antibody/rabbit+leucine+enkephalin+antibody/pmc03606949-49-26-32
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pro dynorphin  (Neuromics)
86
Neuromics pro dynorphin
Ambulation was recorded for 180 min after injection of a single dose of cocaine (25 mg/kg, i.p.) or vehicle in fb-A 2A R KO (n = 12, A ) and in st-A 2A R KO (n = 9, B ) mice and their WT littermates (n = 8–12). The arrow indicates the time of injection and the data are mean ± SEM; *p<0.05, comparing fb-A 2A R KO and st-A 2A R KO groups to their corresponding WT group using two-way ANOVA and a post hoc Bonferroni test. ( C ) Cocaine-induced c-Fos expression in the striatum of fb-A 2A R KO (n = 12) and st-A 2A R KO (n = 9) and their corresponding WT littermates (n = 8–12) # p<0.05, comparing to corresponding wild-types with cocaine treatment, two-way ANOVA post hoc Bonferroni test. ( D ) Representative co-immunostaining of c-Fos with <t>dynorphin.</t> Black arrows indicate neurons co-stained with dynorphin and c-Fos; white arrow heads indicate neurons stained with dynorphin only (greyish brown) and black arrow heads indicate neurons stained with c-Fos (reddish brown). Scale bar = 25 µm.
Pro Dynorphin, supplied by Neuromics, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+anti-dynorphin+polyclonal+antibody/proDynorphin+(GP+Reactive)/pmc03775932-68-37-39
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rabbit anti-grpr antibody  (Cloud-Clone corp)
90
Cloud-Clone corp rabbit anti-grpr antibody
Effect of EA at different frequencies on the expression of <t>GRPR</t> protein in the cervical cord. (A) Representative gel image shows the protein level of GRPR in the control, AEW, 2 Hz EA, 15 Hz EA, 100 Hz EA, and sham EA groups. (B) Summary data show the percentage change in GRPR protein level in six <t>groups.</t> <t>Tubulin</t> (55 kDa) was used as a loading control. The protein band at 43 kDa corresponds to the GRPR protein. Data are expressed as mean ± SEM ( n = 6 mice in each group). * P < 0.05, compared with the control group; # P < 0.05, compared with the sham EA group; + P < 0.05, compared with the 100 Hz EA group.
Rabbit Anti Grpr Antibody, supplied by Cloud-Clone corp, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+anti-dynorphin+polyclonal+antibody/rabbit+anti+grpr+antibody/pmc07921323-80-13-18
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rabbit abs against β-endorphin antibody  (Bachem)
90
Bachem rabbit abs against β-endorphin antibody
Effect of EA at different frequencies on the expression of <t>GRPR</t> protein in the cervical cord. (A) Representative gel image shows the protein level of GRPR in the control, AEW, 2 Hz EA, 15 Hz EA, 100 Hz EA, and sham EA groups. (B) Summary data show the percentage change in GRPR protein level in six <t>groups.</t> <t>Tubulin</t> (55 kDa) was used as a loading control. The protein band at 43 kDa corresponds to the GRPR protein. Data are expressed as mean ± SEM ( n = 6 mice in each group). * P < 0.05, compared with the control group; # P < 0.05, compared with the sham EA group; + P < 0.05, compared with the 100 Hz EA group.
Rabbit Abs Against β Endorphin Antibody, supplied by Bachem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+anti-dynorphin+polyclonal+antibody/rabbit+abs+against+%CE%B2+endorphin+antibody/pmc02631295-198-35-43
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Image Search Results


Ambulation was recorded for 180 min after injection of a single dose of cocaine (25 mg/kg, i.p.) or vehicle in fb-A 2A R KO (n = 12, A ) and in st-A 2A R KO (n = 9, B ) mice and their WT littermates (n = 8–12). The arrow indicates the time of injection and the data are mean ± SEM; *p<0.05, comparing fb-A 2A R KO and st-A 2A R KO groups to their corresponding WT group using two-way ANOVA and a post hoc Bonferroni test. ( C ) Cocaine-induced c-Fos expression in the striatum of fb-A 2A R KO (n = 12) and st-A 2A R KO (n = 9) and their corresponding WT littermates (n = 8–12) # p<0.05, comparing to corresponding wild-types with cocaine treatment, two-way ANOVA post hoc Bonferroni test. ( D ) Representative co-immunostaining of c-Fos with dynorphin. Black arrows indicate neurons co-stained with dynorphin and c-Fos; white arrow heads indicate neurons stained with dynorphin only (greyish brown) and black arrow heads indicate neurons stained with c-Fos (reddish brown). Scale bar = 25 µm.

Journal: PLoS ONE

Article Title: Adenosine A 2A Receptors in Striatal Glutamatergic Terminals and GABAergic Neurons Oppositely Modulate Psychostimulant Action and DARPP-32 Phosphorylation

doi: 10.1371/journal.pone.0080902

Figure Lengend Snippet: Ambulation was recorded for 180 min after injection of a single dose of cocaine (25 mg/kg, i.p.) or vehicle in fb-A 2A R KO (n = 12, A ) and in st-A 2A R KO (n = 9, B ) mice and their WT littermates (n = 8–12). The arrow indicates the time of injection and the data are mean ± SEM; *p<0.05, comparing fb-A 2A R KO and st-A 2A R KO groups to their corresponding WT group using two-way ANOVA and a post hoc Bonferroni test. ( C ) Cocaine-induced c-Fos expression in the striatum of fb-A 2A R KO (n = 12) and st-A 2A R KO (n = 9) and their corresponding WT littermates (n = 8–12) # p<0.05, comparing to corresponding wild-types with cocaine treatment, two-way ANOVA post hoc Bonferroni test. ( D ) Representative co-immunostaining of c-Fos with dynorphin. Black arrows indicate neurons co-stained with dynorphin and c-Fos; white arrow heads indicate neurons stained with dynorphin only (greyish brown) and black arrow heads indicate neurons stained with c-Fos (reddish brown). Scale bar = 25 µm.

Article Snippet: For this procedure, the first antibody, i.e. , a goat anti-dynorphin polyclonal antibody (1:200, sc-46313, Santa Cruz, CA, USA) was detected first, using immunoperoxidase staining enhanced with 0.08% nickel ammonium sulfate, which yields a dark grayish color.

Techniques: Injection, Expressing, Immunostaining, Staining

Effect of EA at different frequencies on the expression of GRPR protein in the cervical cord. (A) Representative gel image shows the protein level of GRPR in the control, AEW, 2 Hz EA, 15 Hz EA, 100 Hz EA, and sham EA groups. (B) Summary data show the percentage change in GRPR protein level in six groups. Tubulin (55 kDa) was used as a loading control. The protein band at 43 kDa corresponds to the GRPR protein. Data are expressed as mean ± SEM ( n = 6 mice in each group). * P < 0.05, compared with the control group; # P < 0.05, compared with the sham EA group; + P < 0.05, compared with the 100 Hz EA group.

Journal: Frontiers in Neuroscience

Article Title: 100 Hz Electroacupuncture Alleviated Chronic Itch and GRPR Expression Through Activation of Kappa Opioid Receptors in Spinal Dorsal Horn

doi: 10.3389/fnins.2021.625471

Figure Lengend Snippet: Effect of EA at different frequencies on the expression of GRPR protein in the cervical cord. (A) Representative gel image shows the protein level of GRPR in the control, AEW, 2 Hz EA, 15 Hz EA, 100 Hz EA, and sham EA groups. (B) Summary data show the percentage change in GRPR protein level in six groups. Tubulin (55 kDa) was used as a loading control. The protein band at 43 kDa corresponds to the GRPR protein. Data are expressed as mean ± SEM ( n = 6 mice in each group). * P < 0.05, compared with the control group; # P < 0.05, compared with the sham EA group; + P < 0.05, compared with the 100 Hz EA group.

Article Snippet: The membrane was incubated with rabbit anti-Dynorphin A antibody (1:1,000; Abcam, Hong Kong), rabbit anti-GRPR antibody (0.75 μg/ml; Cloud-Clone Corp), and horseradish peroxidase (HRP)-conjugated alpha Tubulin Mouse Monoclonal antibody (1:10,000; Proteintech) at 4°C overnight.

Techniques: Expressing

Effect of KOR on the expression of GRPR protein in the cervical cord. (A) Representative gel image shows the protein level of GRPR in the control, AEW, EA + NS, EA + nor-BNI, and sham EA groups. (B) Summary data show the percentage change in GRPR protein level in five groups. Tubulin (55 kDa) was used as a loading control. The protein band at 43 kDa corresponds to the GRPR protein. Data are expressed as mean ± SEM ( n = 6 mice in each group). * P < 0.05, compared with the control group; # P < 0.05, compared with the sham EA group. + P < 0.05, compared with the EA + NS group.

Journal: Frontiers in Neuroscience

Article Title: 100 Hz Electroacupuncture Alleviated Chronic Itch and GRPR Expression Through Activation of Kappa Opioid Receptors in Spinal Dorsal Horn

doi: 10.3389/fnins.2021.625471

Figure Lengend Snippet: Effect of KOR on the expression of GRPR protein in the cervical cord. (A) Representative gel image shows the protein level of GRPR in the control, AEW, EA + NS, EA + nor-BNI, and sham EA groups. (B) Summary data show the percentage change in GRPR protein level in five groups. Tubulin (55 kDa) was used as a loading control. The protein band at 43 kDa corresponds to the GRPR protein. Data are expressed as mean ± SEM ( n = 6 mice in each group). * P < 0.05, compared with the control group; # P < 0.05, compared with the sham EA group. + P < 0.05, compared with the EA + NS group.

Article Snippet: The membrane was incubated with rabbit anti-Dynorphin A antibody (1:1,000; Abcam, Hong Kong), rabbit anti-GRPR antibody (0.75 μg/ml; Cloud-Clone Corp), and horseradish peroxidase (HRP)-conjugated alpha Tubulin Mouse Monoclonal antibody (1:10,000; Proteintech) at 4°C overnight.

Techniques: Expressing